Two and three photon imaging for personalized and real-time treatment optimization

Our poster presentation at FOM 2024

In the realm of biomedical imaging, the quest for techniques that offer both molecular specificity and real-time visualization capabilities has been incessant. We propose simultaneous two and three photon imaging as a transformative approach facilitated by a new class of femtosecond lasers. These lasers boast a broadband emission spectrum, facilitating the simultaneous excitation of multiple fluorophores and harmonics. Additionally, their sub-50 femtosecond pulse duration enables prolonged imaging over days without inducing tissue heating, thus preserving cellular viability and functionality.