Lasers for Fluorescence Microscopy

Fluorescence microscopy basics

Over the last decade, fluorescence based life science research has been revolutionized by new imaging methods and the transitioning from bulky gas-laser sources into solid-state lasers with a smaller footprint, longer lifetime, and lower maintenance requirements.

One of the most important tools for microbiology research is high-resolution live-cell imaging through fluorescence microscopy, in which certain molecules, so-called fluorochromes or fluorophores, return low-energy light after excitation with light of a defined wavelength, i.e. light with a higher wavelength than the excitation light. Scientists are taking advantage of this physical effect to investigate ever smaller structures visible