Breakthroughs using multiphoton microscopy

Multiphoton microscopy has revolutionized biological imaging by allowing high-resolution imaging in 3D without the need for dyes or labels. An important aspect of this technology is the use of very short femtosecond pulses. The use of these pulses has several advantages that have made multiphoton microscopy a valuable tool in the study of biological samples.

One of the major advantages of using femtosecond pulses in multiphoton microscopy is reduced photodamage to the sample. The short pulse duration minimizes the exposure time to light, reducing the amount of photodamage that occurs during imaging. This is particularly important in the study of live samples, as it allows researchers to obtain high-resolution images without affecting the viability of the sample.