Photo damage is considered one of the main limiting factors when it comes to live cell imaging, since constant exposure to high average laser power considerably accelerates phototoxicity.
One practical solution to address this issue is the use of ultrafast laser technologies that can deliver very short femtosecond pulses. The true benefit for multiphoton imaging is even greater with the use of sub 50 fs pulse durations which can deliver exceptional peak powers, produce very bright high contrast multiphoton images while keeping the average laser power low, and thereby prolong cell viability and allow longer imaging times.
In this webinar we present the results of high-resolution label-free imaging through a combination of second- and third-order non-linear imaging and two- and three-photon fluorescence microscopy using a single compact sub-50 fs laser. This innovative approach opens doors to precision tissue examinations, particularly for use in instant pathology.
Join Dr. Oliver Prochnow and Dr. Marloes Groot from Vrije University in Amsterdam as they showcase the remarkable potential of sub-50 fs pulses for enhanced multiphoton microscopy.
