Multi-line Lasers simplify fluorescence microscopy

Fluorescence microscopy instrumentation relies on illumination sources to excite fluorophores. Common illumination sources are LEDs, super-continuum white-light sources, or single-wavelength lasers.Lasers are primarily used for high-resolution and high-throughput imaging techniques, and each wavelength excites a different set of fluorophores. In order to efficiently excite multiple fluorophores, it is necessary to use many single-wavelength lasers in one instrument or experiment.  This strengthens the content and quality of results. Along with the advantage of activating more fluorophores comes the challenge of integrating each of the individual wavelengths required.

Currently, many researchers and manufacturers align and integrate individual laser sources for each wavelength on the optical bench or in instrument. These laser combiners and laser light engines have simplified some of these assemblies substantially. However, they do not eliminate the need for alignment (and re-alignment) over time. A permanently aligned multi-line laser offers a robust and alignment free option.

Simplifying imaging with the Cobolt Skyra

The Cobolt Skyra multi-line laser with its unique design and manufacturing offers a compelling solution to the challenges of permanent beam alignment. This system delivers the functionality of multiple wavelengths in one easy-to-use compact unit, making it ideal for both system integration and laboratory setups. It is built using patent-pending alignment techniques and utilizing Cobolt’s proprietary HTCure technology, which is based on careful thermo-mechanical matching and high-temperature fixation of miniaturized optics. The lasers are built on a single, temperature-controlled platform for stable operation and protection from thermomechanical misalignment.

The Cobolt Skyra can include up to four wavelengths, within the range of 405 nm to 785 nm, including coverage in the green-orange, with beam position overlap <50 μm at the exit and pointing stability <10 μrad/°C over a temperature range of 20 °C to 50 °C. The output of the Cobolt Skyra can be provided as free beam or fiber coupled into SM/PM fiber. All drive and control electronics is fully integrated into the compact laser housing with input connectors for digital and analog modulation conveniently accessible on the back panel.

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