Read our application note on TRAST microscopy for measuring oxygen concentration in cancerous cells.

In the field of biology it is a general consensus that cancerous cells often use other metabolic pathways, than corresponding healthy cells, and thereby consume less oxygen. If it is possible to measure the oxygen levels of cells by fluorescence microscopy this could be exploited as a future tool in clinical cancer diagnosis. However, measuring oxygen concentration of live cells is not totally straight forward. Traditional fluorophores have too short excited state lifetimes (nanoseconds) to be significantly influenced by molecular oxygen collisions, typically taking place in the microsecond time range.

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