On the 16th of February 2023 we hosted the years first Optopub with Photonics Sweden where we combined the event with a soft opening of our new office expansion.
Thanks to PhotonicSweden for the opportunity and to all who came to listen to invited speakers, see our new office area and enjoy some dinner. We look forward to the next Optopub event soon!
Real-time single-molecule 3D tracking in E.Coli
Presented by Elias Amselem, Department of Cell and Molecular Biology, Uppsala University
We have little detailed knowledge about the intracellular dynamics of fundamental processes such as gene regulation or protein translation; the intracellular environment is very different from the dilute conditions where most of what we know about biochemistry has been learned. Here, we present a 3D single-molecule tracking system based on cross ¼ entropy. Putting the system into practice, we track single fluorophores within the Escherichia coli bacterium. A fluorescent marker is attached to the E. coli trigger factor (TF), a bacterial chaperone protein that binds to translating ribosomes close to the peptide exit tunnel. The system can locate a reporter in a volume of size 0.95×0.95×1.40 of 0.84 ms at a photon count rate of 120kHz
Transient State (TRAST) Spectrocscopy and Imaging of Cellular and Molecular States and Conditions – Exploiting the Sensing Side of Fluorophore Blinking Kinetics
Presented by Jerker Widengren, Exp Biomol. Physics group, Dept. Applied Physics, Royal Institute of Technology (KTH)
Dark state transitions of fluorophores are central for all forms of fluorescence-based, single-molecule and super-resolution microscopy and spectroscopy, mainly as limiting factors in single molecule studies, at the same time prerequisites for super-resolution imaging techniques. This overview will highlight an additional aspect of such transitions, namely that they can be used to sense a manifold of biomolecular environments, dynamics and interactions. Principles for how they can be imaged will be described, as well as application examples in cells and tissues.
The B-Brighter Project
Dr. Gustaf Mårtensson, Expert Complex Fluids at Mycronic, Adjunct researcher at CBH-KTH
The B-BRIGHTER project is an EU-financed research project with the goal of developing a novel, light-based bioprinting technology. The project utilises continuous-wave lasers, acousto-optics, galvanomirrors and custom hydrogel-based bioinks to make this concept a reality.
HÜBNER PHOTONICS offers a full range of high performance lasers and terahertz systems including single and multi-line Cobolt lasers, tunable C-WAVE lasers, C-FLEX laser combiners, VALO femtosecond fiber lasers and Terahertz imagers and spectrometers.
Through supply of innovative and reliable products, dedicated customer care and a certified quality management system, HÜBNER Photonics has become a preferred supplier to major instrument manufacturers, leading research groups and public institutions working in the areas of spectroscopy, bioinstrumentation, holography, metrology, particle analysis, quantum technology and public security.