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A Multi-line laser is a single compact laser head with multiple lasers built inside and collimated into a single permanently aligned output beam, thus giving the appearance of a single laser with multiple wavelengths. The multi-line laser is therefore very attractive as an extremely compact, an easy to use solution for adding more functionality and wavelengths either to a system design or lab set-up. It is the simplicity and compactness which makes the multi-line laser very attractive for use in light sheet microscopy.

Lasers are primarily used for high-resolution and high-throughput imaging techniques, and each wavelength excites a different set of fluorophores. In order to efficiently excite multiple fluorophores, it is necessary to use many single-wavelength lasers in one instrument or experiment. Read how our multi-line laser helps make life simpler in instrument design as well as the lab.

A simplified solution for integrating multiple laser wavelengths into a fluorescence microscope is to use a multi-line laser solution. It is now possible to deliver up to four laser colors from one compact and permanently aligned laser package. Read about how it works here.

In the field of biology it is a general consensus that cancerous cells often use other metabolic pathways, than corresponding healthy cells, and thereby consume less oxygen. Read our application note on TRAST microscopy for measuring oxygen concentration in cancerous cells.

The Cobolt Rogue™ 640 nm is multi-longitudinal mode in a perfect TEM00 beam with 1 W output power, ideally suited for super resolution STORM microscopy.