Brillouin Spectrum of a Bovine Bone

The mechanical properties of a polished bovine bone were optically probed using the LightMachinery Brillouin system. This technique analyzes the inelastic scattering of photons following interactions with acoustic phonons in a material.

A Brillouin confocal microscope (HF-9000) with a 20X/0.4NA objective was employed to focus 20 mW of laser power (Cobolt 04-01 Series, λ = 532 nm) on the sample.